ACV-HM001-100μg / 询价
ACV-HM001-500μg / 询价
ACV-HM001-500μgx2 / 询价
Human Activin A Protein
Recombinant Human Activin A Protein is expressed from HEK293 without tag.It contains Gly311-Ser426 [Accession | P08476].
The protein has a predicted MW of 12.97 kDa. Due to glycosylation, the protein migrates to 14-15 kDa under reduced (R) condition, and 23-25 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
Less than 0.1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in 4mM HCI. Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 4mM HCI.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human Activin A on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.
Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is < 7 ng/mL (QC Test).
Immobilized Human Activin A, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human Activin RIIB, His Tag with the EC50 of 21.4ng/ml determined by ELISA (QC Test).
Immobilized Human Activin A, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human Activin RIIA, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA.
Biotinylated Human Activin RIIB, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human Activin A, No Tag with an affinity constant of 55.35 pM as determined in SPR assay (Biacore T200).
Activin-A, a member of the TGF-β superfamily, Extensive research over the past decades illuminated fundamental roles for activin-A in essential biologic processes, including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis. Activin-A signals through two type I and two type II receptors which, upon ligand binding, activate their kinase activity, phosphorylate the SMAD2 and 3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and activate or silence gene expression.
INHBA; Activin A; Inhibin beta A chain;
Morianos I, Papadopoulou G, Semitekolou M, Xanthou G. Activin-A in the regulation of immunity in health and disease. J Autoimmun. 2019 Nov;104:102314. doi: 10.1016/j.jaut.2019.102314. Epub 2019 Aug 13. PMID: 31416681.
